instrumented BioBLU^® 5c stirred bioreactor are recommended.

The growth of cells in the spinner flasks can be described with an

accuracy of approximately 80% using the model developed by Jos-

sen [6] and can therefore also be applied to cultivations undertaken

in the BioBLU^® 5c.

As exemplified by the results of Fig. 3a, the recorded cell

growth is almost identical in both cultivation systems, with the

BioBLU^® 0.3c achieving a maximum viable cell density of

Fig. 3 Exemplary, as well as modeled results, of the hASC expansion in 125-mL spinner flasks (n ¼ 3) and the

BioBLU^® 0.3c: (a) the viable cell density per mL (χS) and viable cell density per cm² (χA) of the cells during

expansion in the BioBLU^® 0.3c (left) and spinner flasks (right). The double-headed arrow represents a 50%

media exchange 4 d post-inoculation. (b) Changes in glucose (Glc), lactate (Lac) and ammonium (Amn)

concentrations during the 7 days cultivation process. (c) Marker expression rates as a percentage of the total

singlet cell population isolated from both cultivation vessel types and analyzed by flow cytometry and (d)

images taken by microscope after staining with 4⁰,6-diamidino-2-phenylindole (DAPI) to determine cell

attachment and distribution, following a 7-days expansion period

Mesenchymal Stem Cell Expansion at Benchtop-Scale

91